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Image Search Results
Journal: Journal of Lipid Research
Article Title: Characterization of the small molecule ARC39, a direct and specific inhibitor of acid sphingomyelinase in vitro
doi: 10.1194/jlr.RA120000682
Figure Lengend Snippet: Toxicity of ARC39 in vitro. (A, B) L929 and HepG2 cells were treated with ARC39 as indicated. A: After 24 h treatment, cells were stained with annexin V/PI and analyzed by flow cytometry. B: After 24 and 48 h treatment, cell viability was determined with XTT reduction assay. C, D: Unprenylated Rap1A and RAP1A protein, respectively, normalized to β-actin in L929 and HepG2 cells, respectively, after 24 h treatment with ARC39 with representative Western blots. Zoledronate served as a positive control. Control values are normalized to their mean. Data are represented as mean ± SD, n = 3–4 experiments. One-way ANOVA was used in A, C, and D and two-way ANOVA in B, both followed by Bonferroni correction. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.
Article Snippet: Proteins were transferred to nitrocellulose membranes blocked 1 h with StartingBlock blocking buffer (Thermo Fisher) and incubated with the appropriate primary antibody overnight at 4°C: anti-AC (4741, ProSci, 1:1,000), anti-ASM (AF5348, R&D, 1:50),
Techniques: In Vitro, Staining, Flow Cytometry, Western Blot, Positive Control, Control
Journal: Genome Biology
Article Title: Identification of secondary targets of N-containing bisphosphonates in mammalian cells via parallel competition analysis of the barcoded yeast deletion collection
doi: 10.1186/gb-2009-10-9-r93
Figure Lengend Snippet: N-BP-induced accumulation of unprenylated Rap1A and increase of DBF4, but not of TBCB, can be reversed by GGPP. Western blot (WB) analysis of MCF-7 cells treated with 10 -4 M ALE alone or in combination with 25 μM GGPP. The same volume of absolute ethanol was used as control vehicle of GGPP (Ctrl). Actin was used to show equal loading of the lanes.
Article Snippet: Blots were incubated with the following polyclonal antibodies: rabbit anti-Dbf4 (Santa Cruz Biotechnology Inc., Santa Cruz, CA, USA), rabbit anti-actin (Sigma),
Techniques: Western Blot